5K5J

Homo sapiens CCCTC-binding factor (CTCF) ZnF5-8 and DNA complex structure in space group P41212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-10
Detector	RAYONIX MX300-HS
Wavelength(s)	1.000
Spacegroup name	P 41 21 2
Unit cell lengths	58.903, 58.903, 172.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.867 - 2.287
R-factor	0.2102
Rwork	0.208
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5k5i
RMSD bond length	0.004
RMSD bond angle	0.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((dev_2400: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.867	2.480
High resolution limit [Å]	2.287	2.390
Rmerge	0.080	0.965
Number of reflections	14269
<I/σ(I)>	28.271	2.7
Completeness [%]	99.9	100
Redundancy	10.8	11
CC(1/2)	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	25% (w/v) polyethylene glycol 3350, 0.2 M ammonium sulfate, and 0.1 M bis-tris HCl, pH 5.5