5K5H
Homo sapiens CCCTC-binding factor (CTCF) ZnF4-7 and DNA complex structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-26 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.27046 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 52.800, 52.800, 166.170 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.321 - 3.108 |
R-factor | 0.2395 |
Rwork | 0.236 |
R-free | 0.26350 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.454 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX ((dev_2400: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.321 | 3.190 |
High resolution limit [Å] | 3.108 | 3.108 |
Rmerge | 0.088 | 0.059 |
Number of reflections | 44172 | |
<I/σ(I)> | 17.56 | |
Completeness [%] | 70.8 | 4.7 |
Redundancy | 7.7 | 7.9 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 25% (w/v) polyethylene glycol 3350, 0.2 M ammonium acetate, 0.1 M bis-tris HCl, pH 5.5 |