5K51
Trypanosome brucei Hypoxanthine-guanine phosphoribosyltranferase in complex with a 9-[5-(phosphonoheptyl]hypoxanthine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.093, 88.633, 94.664 |
| Unit cell angles | 90.00, 107.16, 90.00 |
Refinement procedure
| Resolution | 46.460 - 2.956 |
| R-factor | 0.251 |
| Rwork | 0.246 |
| R-free | 0.29420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jsq |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.468 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.460 | 3.140 |
| High resolution limit [Å] | 2.956 | 2.960 |
| Rmerge | 0.142 | 0.539 |
| Number of reflections | 18891 | |
| <I/σ(I)> | 5.9 | 1.6 |
| Completeness [%] | 99.6 | 95 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 25% PEG 3350, 0.2 M sodium iodide, 0.1 M Bis-Tris propane |
