5JXY
Enzyme-substrate complex of TDG catalytic domain bound to a G/U analog
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97947 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.450, 53.270, 82.540 |
| Unit cell angles | 90.00, 95.63, 90.00 |
Refinement procedure
| Resolution | 19.700 - 1.710 |
| R-factor | 0.1995 |
| Rwork | 0.198 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ufj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 19.700 |
| High resolution limit [Å] | 1.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 0.2M Ammonium Chloride and 20% PEG3350 |
