5JXE
Human PD-1 ectodomain complexed with Pembrolizumab Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-01-27 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.966 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 105.554, 105.554, 380.048 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.926 | 3.080 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.120 | |
Number of reflections | 28657 | |
<I/σ(I)> | 9 | 1.5 |
Completeness [%] | 99.4 | 99.2 |
Redundancy | 4.3 | 4.2 |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M sodium citrate pH 5.6, 19 mM n-Decyl-N,N-dimethylglycine, 20% isopropanol and 20% PEG 4000 |