5JWE
Crystal structure of H-2Db in complex with the LCMV-derived GP92-101 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.500, 176.300, 85.600 |
| Unit cell angles | 90.00, 119.80, 90.00 |
Refinement procedure
| Resolution | 19.450 - 2.400 |
| R-factor | 0.2024 |
| Rwork | 0.199 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n5a |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.241 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.610 | 2.430 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.070 | 0.690 |
| Number of reflections | 84550 | |
| <I/σ(I)> | 12.7 | 1.8 |
| Completeness [%] | 94.2 | 91 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 100 mM Ammonium Sulfate, 100 mM Tris-HCl, 25% PEG 6000 |
