5JR7
Crystal structure of an ACRDYS heterodimer [RIa(92-365):C] of PKA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2014-05-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.146, 66.790, 87.932 |
| Unit cell angles | 101.76, 89.37, 105.27 |
Refinement procedure
| Resolution | 49.110 - 3.560 |
| R-factor | 0.269 |
| Rwork | 0.266 |
| R-free | 0.32200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qcs |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.738 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.560 | 3.560 |
| Rmerge | 0.155 | 0.490 |
| Number of reflections | 15767 | |
| <I/σ(I)> | 11.8 | 4.1 |
| Completeness [%] | 98.6 | 98.2 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 0.2 M Sodium thiocyanate and 20% PEG 3350 with the protein at a final concentration of 5 mg/ml in a 1.6 ul drop |
