5JR2
Crystal structure of the EphA4 LBD in complex with APYd3 peptide inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-12-04 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.137, 84.467, 127.894 |
| Unit cell angles | 90.00, 93.87, 90.00 |
Refinement procedure
| Resolution | 28.920 - 1.750 |
| R-factor | 0.17209 |
| Rwork | 0.170 |
| R-free | 0.20416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4w4z |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.591 |
| Data scaling software | XDS (March 1, 2015) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.920 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.042 | 0.316 |
| Number of reflections | 78825 | |
| <I/σ(I)> | 17.9 | 3.1 |
| Completeness [%] | 99.3 | 96.5 |
| Redundancy | 3.6 | 3.1 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M MgCl2, 0.1 M Bis-Tris pH 6.5, 25% PEG 3,350, 3% 1,6-hexanediol |
