5JO3
PDE5A for NaV1.7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2010-03-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 55.840, 76.100, 80.920 |
| Unit cell angles | 90.00, 102.56, 90.00 |
Refinement procedure
| Resolution | 44.000 - 1.490 |
| R-factor | 0.18107 |
| Rwork | 0.180 |
| R-free | 0.20141 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.137 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BUSTER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.570 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.115 | |
| Number of reflections | 45627 | |
| <I/σ(I)> | 12.5 | 5 |
| Completeness [%] | 84.8 | 48.8 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 293 | 20% PEG 4K 10% Isopropanol 0.1M Hepes pH 7.4 |
