5JKK
Crystal structure of the negatively supercharged variant Ftn(neg) of human heavy chain ferritin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-15 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 180.980, 180.980, 180.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.370 - 1.600 |
| R-factor | 0.1247 |
| Rwork | 0.124 |
| R-free | 0.14290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cei |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.164 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.370 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.047 | 0.194 |
| Number of reflections | 255788 | |
| <I/σ(I)> | 40.93 | 11.21 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 13.17 | 11.16 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.52 M magnesium acetate, 100 mM Tris, pH 8.5, 8 mg/mL Ftn(neg) |
