5JDO

T. congolense haptoglobin-haemoglobin receptor in complex with haemoglobin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 2 21 21
Unit cell lengths	72.750, 127.300, 172.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	102.410 - 3.200
R-factor	0.206
Rwork	0.204
R-free	0.23510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4E40 and 1HH0
RMSD bond length	0.010
RMSD bond angle	1.210
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	102.410
High resolution limit [Å]	3.200
Rpim	0.073
Number of reflections	26536
<I/σ(I)>	7.3
Completeness [%]	99.0
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M sodium citrate pH 5, 8% w/v PEG 8000