5JCP

RhoGAP domain of ARAP3 in complex with RhoA in the transition state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	56.786, 93.208, 164.283
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.711 - 2.100
R-factor	0.1885
Rwork	0.186
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jd0 1a2b
RMSD bond length	0.007
RMSD bond angle	0.910
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.2)
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.108	0.066	0.506
Number of reflections	52079
<I/σ(I)>	9.5
Completeness [%]	99.8	99.3	99.9
Redundancy	6	5.7	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	100 mM MES, 15%(v/v) PEG 550 MME, 4%(v/v) Acetone