5IUK
Crystal structure of the DesK-DesR complex in the phosphotransfer state with high Mg2+ (150 mM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.930, 115.610, 91.590 |
| Unit cell angles | 90.00, 116.71, 90.00 |
Refinement procedure
| Resolution | 39.270 - 2.900 |
| R-factor | 0.198 |
| Rwork | 0.195 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data scaling software | XDS (Jun 17, 2015) |
| Phasing software | PHASER (2.5.7) |
| Refinement software | BUSTER-TNT (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.780 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.116 | 1.471 |
| Number of reflections | 36132 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 99.1 | 99.2 |
| Redundancy | 5.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 291 | PEG3350, tri-potassium citrate, vapor diffusion, hanging drop, temperature 291K |
