5IR3
Crystal structure of the recombinant highest fibrillogenic natural mutant (obtained from patient AR) derived from lambda 6 light chain variable domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-03-28 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 71.121, 71.121, 96.453 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.810 - 1.700 |
| R-factor | 0.176 |
| Rwork | 0.174 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3b5g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.971 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((DEV_2313)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.810 | 1.770 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.089 | 0.486 |
| Number of reflections | 13830 | |
| <I/σ(I)> | 11.3 | 3.3 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 4.3 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.1M Sodium cacodylate pH 6.5; 1.4M Sodium acetate |
