5IL0

Crystal structural of the METTL3-METTL14 complex for N6-adenosine methylation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9200
Spacegroup name	P 41 21 2
Unit cell lengths	101.695, 101.695, 116.478
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.272 - 1.882
R-factor	0.1763
Rwork	0.175
R-free	0.20500
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.879
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.880
Number of reflections	50152
<I/σ(I)>	37.5
Completeness [%]	99.9
Redundancy	14.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	291	PEG 8000, sodium citrate