5IKN

Crystal Structure of the T7 Replisome in the Absence of DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-25
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	174.866, 238.091, 243.466
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.983 - 4.802
R-factor	0.2768
Rwork	0.275
R-free	0.31780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ajq 1cr0 1nui
RMSD bond length	0.004
RMSD bond angle	0.736
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.983	4.880
High resolution limit [Å]	4.800	4.800
Number of reflections	41183
<I/σ(I)>	10.1
Completeness [%]	97.9	97.6
Redundancy	3.7	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	1.47-1.62M Sodium Formate 0.1M Tris pH 7.5