5IFS
Quantitative interaction mapping reveals an extended ubiquitin regulatory domain in ASPL that disrupts functional p97 hexamers and induces cell death
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-04-27 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.844, 132.914, 96.121 |
| Unit cell angles | 90.00, 110.15, 90.00 |
Refinement procedure
| Resolution | 33.770 - 2.460 |
| R-factor | 0.20352 |
| Rwork | 0.201 |
| R-free | 0.25061 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cf3 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.096 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.800 | 2.520 | |
| High resolution limit [Å] | 2.460 | 11.000 | 2.460 |
| Rmerge | 0.050 | 0.011 | 0.461 |
| Number of reflections | 58583 | ||
| <I/σ(I)> | 16.72 | 61.05 | 2.09 |
| Completeness [%] | 97.9 | 90.2 | 93.2 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 23% PEG3350, 0.3M LiSO4, 0.1M Hepes pH 7.0 |
