5IES

Crystal structure of VRC01c-HuGL2 Fab from an HIV-1 naive donor in complex with with a germline-targeting gp120 engineered outer domain eOD-GT8 at 2.16 A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	293
Detector technology	PIXEL
Collection date	2015-05-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	I 2 2 2
Unit cell lengths	102.553, 138.604, 147.896
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.448 - 2.160
R-factor	0.1906
Rwork	0.189
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jpk
RMSD bond length	0.006
RMSD bond angle	0.782
Data reduction software	HKL-2000 (v706e)
Data scaling software	HKL-2000 (v706e)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX ((1.10_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.450	2.193
High resolution limit [Å]	2.154	2.160
Rmerge		0.665
Number of reflections	52709
<I/σ(I)>	16.2	1.9
Completeness [%]	92.5	89.6
Redundancy	3.9	3.5
CC(1/2)	0.902

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293.15	0.1 M citric acid (pH 4.0), 20% (w/v) PEG6000 with an overall pH 5.0 of the solution, cryo-protected with 25% glycerol