5ICV

Crystal structure of human NatF (hNaa60) bound to a bisubstrate analogue

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 41
Unit cell lengths	48.448, 48.448, 148.368
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.564 - 1.530
R-factor	0.1912
Rwork	0.191
R-free	0.20840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lx9
RMSD bond length	0.007
RMSD bond angle	1.284
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.564	1.568
High resolution limit [Å]	1.530	1.530
Rmerge	0.122	0.635
Number of reflections	51410
<I/σ(I)>	18.62
Completeness [%]	99.8	99.9
Redundancy	13	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	4% Tascimate pH 4.0 and 12% PEG 3500