5HZK
Crystal structure of photoinhibitable Intersectin1 containing wildtype LOV2 domain in complex with Cdc42
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-27 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.97903 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 112.370, 119.080, 131.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.070 | 0.524 |
Number of reflections | 27085 | |
<I/σ(I)> | 23.3 | 3.8 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.6 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6 | 293 | 0.1 M MES, 20% (w/v) PEG 6000 |