5HZK

Crystal structure of photoinhibitable Intersectin1 containing wildtype LOV2 domain in complex with Cdc42

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Cell division control protein 42 homolog	polymer	190	21083.2	2	UniProt (P60953) Pfam (PF00071)	Homo sapiens (Human)	G25K GTP-binding protein
2	B, D (B, D)	Intersectin-1,NPH1-1,Intersectin-1	polymer	502	58281.1	2	UniProt (Q15811) UniProt (O49003) Pfam (PF00621) Pfam (PF16652)	Homo sapiens (Human)	SH3 domain-containing protein 1A,SH3P17,SH3 domain-containing protein 1A,SH3P17
3	E, G (A, C)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537)
4	F, H (B, D)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN) PubChem (710) PubChem (8965) PubChem (643976) PubChem (5702760) PubChem (7048779) PubChem (7048781) PubChem (23585816) PubChem (51435500) PubChem (51435503) PubChem (15559251)

Sequence modifications

A, C: 1 - 188 (UniProt: P60953)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag
Ser 188	Cys 188	engineered mutation

B, D: 1230 - 1308 (UniProt: Q15811)
B, D: 1309 - 1451 (UniProt: O49003)
B, D: 1452 - 1723 (UniProt: Q15811)

PDB	External Database	Details
Leu 1724	-	expression tag
Glu 1725	-	expression tag
His 1726	-	expression tag
His 1727	-	expression tag
His 1728	-	expression tag
His 1729	-	expression tag
His 1730	-	expression tag
His 1731	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	158728.6
Non-Polymers*	Number of molecules	4
	Total formula weight	1799.1
All*	Total formula weight	160527.7

*Water molecules are not included.