5HZH
Crystal structure of photoinhibitable Rac1 containing C450A mutant LOV2 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97885 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.050, 74.270, 58.400 |
Unit cell angles | 90.00, 94.62, 90.00 |
Refinement procedure
Resolution | 37.135 - 2.600 |
R-factor | 0.2063 |
Rwork | 0.204 |
R-free | 0.25530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wkq |
RMSD bond length | 0.002 |
RMSD bond angle | 0.610 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.101 | 0.685 |
Number of reflections | 9516 | |
<I/σ(I)> | 14.3 | 3.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.5 | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 0.2 M calcium acetate, 26% (w/v) PEG 3350 |