5HPT
System-wide modulation of HECT E3 ligases with selective ubiquitin variant probes: WWP1, Ubv P2.3 and UBCH7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-03-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 114.005, 118.897, 158.663 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 95.146 - 2.840 |
R-factor | 0.219 |
Rwork | 0.218 |
R-free | 0.23700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nd7 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.612 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 95.150 |
High resolution limit [Å] | 2.840 |
Number of reflections | 97828 |
<I/σ(I)> | 15.7 |
Completeness [%] | 99.9 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 293 | isopropanol 10%, PEG3350 8%, sodium citrate 0.1 M |