5HPE
Phosphatase domain of PP5 bound to a phosphomimetic Cdc37 substrate peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-03-19 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.968600 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.314, 65.164, 132.117 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.390 - 2.270 |
R-factor | 0.1753 |
Rwork | 0.168 |
R-free | 0.23590 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1s95 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.632 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.390 | 2.340 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.164 | 0.657 |
Number of reflections | 16821 | |
<I/σ(I)> | 9.5 | 3 |
Completeness [%] | 92.0 | 86.8 |
Redundancy | 5.3 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 277 | 18% PEG, 8% ethylene glycol, 0.1M Hepes pH 7.5, 20mM hexamine cobolt chloride |