5H3E
Crystal structure of mouse isocitrate dehydrogenases 2 K256Q mutant complexed with isocitrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2014-12-07 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 32 |
| Unit cell lengths | 58.150, 58.150, 206.915 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.320 - 2.210 |
| R-factor | 0.211 |
| Rwork | 0.209 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4l04 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.300 | 2.340 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Number of reflections | 37204 | |
| <I/σ(I)> | 16 | 3 |
| Completeness [%] | 94.5 | 71.7 |
| Redundancy | 4.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | 0.1 M ammonium formate, 24%(w/v) PEG 3350 |
