5H3A
Structural analysis of KSHV thymidylate synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9797 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.410, 79.539, 67.283 |
| Unit cell angles | 90.00, 116.24, 90.00 |
Refinement procedure
| Resolution | 19.917 - 2.400 |
| R-factor | 0.1812 |
| Rwork | 0.178 |
| R-free | 0.23220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h38 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.330 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.365 | 2.400 |
| Number of reflections | 23609 | |
| <I/σ(I)> | 28.4 | 2.7 |
| Completeness [%] | 98.7 | 98.5 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 300 | potassium phosphate (monobasic), PEG 8K, glycerol |
