5H1D

Crystal structure of C-terminal of RhoGDI2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	55.020, 55.020, 96.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.340 - 1.494
R-factor	0.1564
Rwork	0.154
R-free	0.20390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rho
RMSD bond length	0.004
RMSD bond angle	0.709
Data reduction software	iMOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.833	36.340	1.570
High resolution limit [Å]	1.494	4.720	1.494
Rmerge		0.051	0.502
Number of reflections	24846
<I/σ(I)>	18.1	11.7	1.3
Completeness [%]	99.9	95.5	100
Redundancy	13.6	10.7	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% PEG 1500