5GZW
Crystal structure of AmpC BER adenylylated by acetyl-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.98000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.480, 65.039, 177.271 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.678 - 1.489 |
R-factor | 0.2121 |
Rwork | 0.211 |
R-free | 0.23710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3b |
RMSD bond length | 0.007 |
RMSD bond angle | 1.167 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.520 |
High resolution limit [Å] | 1.489 | 1.490 |
Number of reflections | 115016 | |
<I/σ(I)> | 32 | |
Completeness [%] | 98.3 | |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 5.5 | 295 | 0.2M Lithium sulfate, 0.1M Bis-tris pH 5.5, 25% polyethylene glycol 3500 |