5GP7
Structural basis for the binding between Tankyrase-1 and USP25
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97776 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.506, 59.242, 39.995 |
| Unit cell angles | 90.00, 100.68, 90.00 |
Refinement procedure
| Resolution | 39.303 - 1.502 |
| R-factor | 0.1743 |
| Rwork | 0.172 |
| R-free | 0.20720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3twu |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.956 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 25690 | |
| <I/σ(I)> | 10.93 | |
| Completeness [%] | 98.7 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 100 mM HEPES/sodium hydroxide, 200 mM sodium chloride, 20% (w/v) PEG 6000 |
