5GKP
Crystal structure of the EndoG worm homologue CPS-6 H148A/F122A in complex with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 193 |
Detector technology | CCD |
Collection date | 2014-01-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.8 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.672, 72.562, 127.914 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.783 - 2.300 |
R-factor | 0.1801 |
Rwork | 0.173 |
R-free | 0.25250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3s5b |
RMSD bond length | 0.014 |
RMSD bond angle | 1.426 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.350 |
High resolution limit [Å] | 2.300 | 2.270 |
Number of reflections | 25101 | |
<I/σ(I)> | 23.1 | |
Completeness [%] | 99.8 | |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 279 | 0.1 M HEPES, 30% PEG 1000 |