5GGS
PD-1 in complex with pembrolizumab Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-05 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.170, 54.195, 104.039 |
| Unit cell angles | 105.69, 96.99, 96.06 |
Refinement procedure
| Resolution | 30.038 - 1.997 |
| R-factor | 0.1819 |
| Rwork | 0.179 |
| R-free | 0.22800 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.165 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.990 |
| Number of reflections | 71844 |
| <I/σ(I)> | 20.1 |
| Completeness [%] | 95.2 |
| Redundancy | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100 mM MES, pH 6.5, 12% PEG500 MME, 6% PEG20,000, 50 mM ammonium acetate |
