5FZO

Crystal structure of the catalytic domain of human JmjD1C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-30
Detector	DECTRIS PILATUS
Spacegroup name	P 2 21 21
Unit cell lengths	45.320, 110.920, 165.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.460 - 1.840
R-factor	0.1945
Rwork	0.193
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ypd
RMSD bond length	0.008
RMSD bond angle	1.145
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	DIMPLE
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.460	1.890
High resolution limit [Å]	1.840	1.840
Rmerge	0.130	1.500
Number of reflections	72743
<I/σ(I)>	9	1.1
Completeness [%]	99.1	97.6
Redundancy	6.6	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		20% PEG3350 -- 10% ETHYLENE GLYCOL -- 0.1M BIS-TRIS-PROPANE PH 8.5 -- 0.2M POTASSIUM CITRATE TRIBASIC