5FWJ
Crystal structure of human JARID1C in complex with KDM5-C49
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-08 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.960, 67.340, 74.730 |
| Unit cell angles | 86.71, 76.56, 70.35 |
Refinement procedure
| Resolution | 51.232 - 2.100 |
| R-factor | 0.1568 |
| Rwork | 0.155 |
| R-free | 0.18930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a1f |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.953 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1682)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.230 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.080 | 0.270 |
| Number of reflections | 95919 | |
| <I/σ(I)> | 8.24 | 3.37 |
| Completeness [%] | 97.4 | 96 |
| Redundancy | 2.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 0.1M BIS-TRIS PH 5.5 -- 25% PEG3350 -- 0.25M MAGNESIUM CHLORIDE |
