5FUZ
Extending the half-life of a Fab fragment through generation of a humanised anti-Human Serum Albumin (HSA) Fv domain: an investigation into the correlation between affinity and serum half-life
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-05 |
| Detector | DECTRIS PILATUS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 111.210, 111.210, 89.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.680 |
| R-factor | 0.2119 |
| Rwork | 0.212 |
| R-free | 0.25090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | IN-HOUSE FAB |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.421 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.820 |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.120 | 0.360 |
| Number of reflections | 18151 | |
| <I/σ(I)> | 23 | 10.1 |
| Completeness [%] | 99.5 | 99.4 |
| Redundancy | 21.5 | 22.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 2 M DL-MALIC ACID |
