5FTI
Crystal structure of the GluA2 K738M-T744K LBD in complex with glutamate (lithium form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 67.316, 47.562, 96.754 |
| Unit cell angles | 90.00, 95.65, 90.00 |
Refinement procedure
| Resolution | 66.989 - 1.350 |
| R-factor | 0.1615 |
| Rwork | 0.161 |
| R-free | 0.17750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uxa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.391 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.000 | 1.390 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.070 | 1.110 |
| Number of reflections | 126864 | |
| <I/σ(I)> | 10.9 | 1.88 |
| Completeness [%] | 94.7 | 92.5 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 22% PEG 4,000, 200 MM LI SULPHATE, 100MM ACETATE PH 5.0 |
