5FSO
MTH1 substrate recognition: Complex with a methylaminopyrimidinedione.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU SATURN 92 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.313, 66.143, 36.197 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.200 - 1.670 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.21220 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.000 |
| High resolution limit [Å] | 1.670 |
| Rmerge | 0.060 |
| Number of reflections | 14832 |
| Completeness [%] | 85.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 25% (W/V) PEG3350 200MM LITHIUM SULPHATE 100MM SODIUM ACETATE PH4.5 |
