5FQL
Insights into Hunter syndrome from the structure of iduronate-2- sulfatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-13 |
| Detector | ADSC CCD |
| Wavelength(s) | 0.9763, 1.475 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 71.050, 71.050, 285.879 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.525 - 2.300 |
| R-factor | 0.1824 |
| Rwork | 0.181 |
| R-free | 0.20810 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.614 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.520 | 2.390 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.080 | 0.940 |
| Number of reflections | 35738 | |
| <I/σ(I)> | 12.4 | 1.9 |
| Completeness [%] | 96.2 | 97.7 |
| Redundancy | 5.3 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.1 | 0.1 M MES PH=6.1 1.0 M LICL 20% PEG6K |
