5FQD
Structural basis of Lenalidomide induced CK1a degradation by the crl4crbn ubiquitin ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-20 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 87.970, 109.930, 112.400 |
| Unit cell angles | 106.02, 93.19, 101.63 |
Refinement procedure
| Resolution | 65.300 - 2.450 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ei3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.180 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.300 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.080 | 1.500 |
| Number of reflections | 140745 | |
| <I/σ(I)> | 8.7 | 0.8 |
| Completeness [%] | 97.3 | 96.5 |
| Redundancy | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 70 MM TRIS PH 7.0 140 MM MGCL2 7% W/V PEG 8000 |
