5FQ5
Crystal structure of Cas9-sgRNA-DNA complex solved by native SAD phasing
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.740, 67.570, 188.190 |
| Unit cell angles | 90.00, 111.31, 90.00 |
Refinement procedure
| Resolution | 47.906 - 2.136 |
| R-factor | 0.1915 |
| Rwork | 0.191 |
| R-free | 0.22480 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.657 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.140 | 2.140 |
| Rmerge | 0.140 | 1.500 |
| Number of reflections | 113763 | |
| <I/σ(I)> | 28.61 | 0.76 |
| Completeness [%] | 96.7 | 65 |
| Redundancy | 148.1 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 0.1 M TRIS-ACETATE PH 8.5, 0.3 M KSCN, 15% PEG (W/V) 3350 |
