5FMK
Bcl-xL with Bak BH3 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-26 |
| Detector | ADSC Q210R |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.668, 55.459, 61.053 |
| Unit cell angles | 90.00, 127.12, 90.00 |
Refinement procedure
| Resolution | 19.401 - 1.731 |
| R-factor | 0.159 |
| Rwork | 0.158 |
| R-free | 0.18560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p1l |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.400 | 1.810 |
| High resolution limit [Å] | 1.730 | 1.730 |
| Rmerge | 0.040 | 0.300 |
| Number of reflections | 21521 | |
| <I/σ(I)> | 35.2 | 4.73 |
| Completeness [%] | 99.4 | 95.4 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.16M CALCIUM ACETATE, 0.08M SODIUM CACODYLATE, PH6.5, 20% GLYCEROL, 14.4% PEG 8000 |
