5FMJ
Bcl-xL with mouse Bak BH3 Q75L complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-11 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 57.789, 57.789, 268.327 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.662 - 2.430 |
| R-factor | 0.1819 |
| Rwork | 0.180 |
| R-free | 0.21950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p1l |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.935 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.660 | 2.670 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.080 | 0.760 |
| Number of reflections | 10808 | |
| <I/σ(I)> | 27.6 | 3.8 |
| Completeness [%] | 99.3 | 98.3 |
| Redundancy | 19.2 | 14.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M SODIUM THIOCYANATE, 20% PEG 3350 |
