5FIC

Open form of murine Acid Sphingomyelinase in presence of lipid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-08-11
Detector	RAYONIX MX-300
Wavelength(s)	0.97959
Spacegroup name	P 41
Unit cell lengths	101.750, 101.750, 401.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.318 - 2.800
R-factor	0.1912
Rwork	0.189
R-free	0.23560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hnq
RMSD bond length	0.005
RMSD bond angle	0.640
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	49.318
High resolution limit [Å]	2.800
Number of reflections	99971
<I/σ(I)>	9.92
Completeness [%]	100.0
Redundancy	6.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium/potassium phosphate, MES, sodium chloride