5FG4
Crystal structure of the bromodomain of human BRPF1 in complex with OF-1 chemical probe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 47.802, 60.704, 51.171 |
| Unit cell angles | 90.00, 100.43, 90.00 |
Refinement procedure
| Resolution | 25.160 - 1.650 |
| R-factor | 0.20265 |
| Rwork | 0.200 |
| R-free | 0.25416 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lc2 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.589 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.160 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.042 | 0.455 |
| Number of reflections | 17300 | |
| <I/σ(I)> | 18.4 | 3.1 |
| Completeness [%] | 99.4 | 94.2 |
| Redundancy | 5.9 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277.15 | 25% PEG3350, 0.1M bis-tris propane pH 7.4, 5% ethyleneglycol, 0.15M sodium nitrate |
