5FER
Complex of TRIM25 RING with UbcH5-Ub
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.2829 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.480, 71.700, 160.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.295 - 2.340 |
| R-factor | 0.2293 |
| Rwork | 0.227 |
| R-free | 0.27310 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.539 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.280 | 2.420 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.139 | 1.918 |
| Number of reflections | 30000 | |
| <I/σ(I)> | 13.75 | |
| Completeness [%] | 99.3 | |
| Redundancy | 12.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 10% PEG 20000 and 100 mM Tris pH 8.5 |
