5FCW
HDAC8 Complexed with a Hydroxamic Acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-02-18 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.443, 84.548, 94.307 |
Unit cell angles | 90.00, 100.08, 90.00 |
Refinement procedure
Resolution | 49.659 - 1.979 |
R-factor | 0.174 |
Rwork | 0.172 |
R-free | 0.21570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.068 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.670 |
High resolution limit [Å] | 1.900 |
Rmerge | 0.061 |
Number of reflections | 107583 |
<I/σ(I)> | 10.5 |
Completeness [%] | 97.9 |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 298 | 4% PEG 3350, 50 mM buffer (MES, pH 5.3) |