5F4E
Crystal structure of the human sperm Izumo1 and egg Juno complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-24 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.77 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 148.000, 55.800, 81.600 |
Unit cell angles | 90.00, 103.60, 90.00 |
Refinement procedure
Resolution | 47.962 - 2.400 |
R-factor | 0.1822 |
Rwork | 0.180 |
R-free | 0.22170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lrh |
RMSD bond length | 0.010 |
RMSD bond angle | 1.183 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.8) |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.962 | 47.960 | 2.490 |
High resolution limit [Å] | 2.400 | 8.980 | 2.400 |
Rmerge | 0.076 | 0.055 | 0.679 |
Rpim | 0.030 | 0.023 | 0.270 |
Total number of observations | 184910 | 3469 | 18998 |
Number of reflections | 25587 | ||
<I/σ(I)> | 14.4 | 33.6 | 2.8 |
Completeness [%] | 100.0 | 99.4 | 99.8 |
Redundancy | 7.2 | 6.6 | 7.2 |
CC(1/2) | 0.998 | 0.996 | 0.954 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M MES [pH 6.5], 20% (w/v) PEG 4000 and 0.6 M NaCl |