5F1G
Crystal structure of AmpC BER adenylylated in the cytoplasm
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9796 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.478, 65.414, 106.115 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.707 - 1.760 |
R-factor | 0.1726 |
Rwork | 0.172 |
R-free | 0.19230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1c3b |
RMSD bond length | 0.007 |
RMSD bond angle | 1.142 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.760 |
Number of reflections | 42395 |
<I/σ(I)> | 79.18 |
Completeness [%] | 99.6 |
Redundancy | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 3.2 | 295 | LiSO4, Citric Acid, PEG 3350, MgCl2 |