5EWX

Fusion protein of T4 lysozyme and B4 domain of protein A from staphylococcal aureus with chemical cross-linker EY-CBS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	77
Detector technology	CCD
Collection date	2015-10-08
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97940
Spacegroup name	C 1 2 1
Unit cell lengths	89.522, 149.850, 55.836
Unit cell angles	90.00, 122.69, 90.00

Refinement procedure

Resolution	42.745 - 2.600
R-factor	0.2076
Rwork	0.203
R-free	0.25320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lyd 1dee
RMSD bond length	0.010
RMSD bond angle	1.493
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	35397
<I/σ(I)>	18.3
Completeness [%]	99.1
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	277	1.84M Na/K Phosphate, pH 7.5