5EWX

Fusion protein of T4 lysozyme and B4 domain of protein A from staphylococcal aureus with chemical cross-linker EY-CBS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Endolysin,Immunoglobulin G-binding protein A,Endolysin	polymer	227	25212.5	2	UniProt (P00720) UniProt (P38507) Pfam (PF00959) Pfam (PF02216)	Enterobacteria phage T4	Lysis protein,Lysozyme,Muramidase,IgG-binding protein A,Staphylococcal protein A
2	C, D (A, B)	2,2'-ethyne-1,2-diylbis{5-[(chloroacetyl)amino]benzenesulfonic acid}	non-polymer		521.3	2	Chemie (EYC)
3	E, F (A, B)	water	water		18.0	64	Chemie (HOH)

Sequence modifications

A, B: 1 - 35 (UniProt: P00720)

PDB	External Database	Details
Gly 12	Arg 12	see sequence details

A, B: 1218 - 1266 (UniProt: P38507)

PDB	External Database	Details
Val 1218	Ala 212	engineered mutation
Ala 1240	Gly 240	engineered mutation
Cys 1258	Glu 258	engineered mutation
Ala 1261	Lys 261	engineered mutation
Ala 1262	Leu 262	engineered mutation

A, B: 38 - 164 (UniProt: P00720)

PDB	External Database	Details
Gly 1209	-	linker
Gly 1210	-	linker
Gly 1211	-	linker
Gly 1212	-	linker
Ser 1213	-	linker
Gly 1214	-	linker
Gly 1215	-	linker
Gly 1216	-	linker
Gly 1217	-	linker
Ser 1218	-	linker
Cys 40	Asn 40	engineered mutation
Thr 54	Cys 54	engineered mutation
Ala 97	Cys 97	engineered mutation
Arg 137	Ile 137	see sequence details
Ala 162	Lys 162	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	50424.9
Non-Polymers*	Number of molecules	2
	Total formula weight	1042.7
All*	Total formula weight	51467.6

*Water molecules are not included.