5EWX
Fusion protein of T4 lysozyme and B4 domain of protein A from staphylococcal aureus with chemical cross-linker EY-CBS
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Endolysin,Immunoglobulin G-binding protein A,Endolysin | polymer | 227 | 25212.5 | 2 | UniProt (P00720) UniProt (P38507) Pfam (PF00959) Pfam (PF02216) In PDB | Enterobacteria phage T4 | Lysis protein,Lysozyme,Muramidase,IgG-binding protein A,Staphylococcal protein A |
2 | A, B | 2,2'-ethyne-1,2-diylbis{5-[(chloroacetyl)amino]benzenesulfonic acid} | non-polymer | 521.3 | 2 | Chemie (EYC) | |||
3 | water | water | 18.0 | 64 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 35 (UniProt: P00720)
A, B: 1218 - 1266 (UniProt: P38507)
A, B: 38 - 164 (UniProt: P00720)
PDB | External Database | Details |
---|---|---|
Gly 12 | Arg 12 | see sequence details |
PDB | External Database | Details |
---|---|---|
Val 1218 | Ala 212 | engineered mutation |
Ala 1240 | Gly 240 | engineered mutation |
Cys 1258 | Glu 258 | engineered mutation |
Ala 1261 | Lys 261 | engineered mutation |
Ala 1262 | Leu 262 | engineered mutation |
PDB | External Database | Details |
---|---|---|
Gly 1209 | - | linker |
Gly 1210 | - | linker |
Gly 1211 | - | linker |
Gly 1212 | - | linker |
Ser 1213 | - | linker |
Gly 1214 | - | linker |
Gly 1215 | - | linker |
Gly 1216 | - | linker |
Gly 1217 | - | linker |
Ser 1218 | - | linker |
Cys 40 | Asn 40 | engineered mutation |
Thr 54 | Cys 54 | engineered mutation |
Ala 97 | Cys 97 | engineered mutation |
Arg 137 | Ile 137 | see sequence details |
Ala 162 | Lys 162 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 50424.9 | |
Non-Polymers* | Number of molecules | 2 |
Total formula weight | 1042.7 | |
All* | Total formula weight | 51467.6 |