5EV1
Structure I of Intact U2AF65 Recognizing a 3' Splice Site Signal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97945 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.052, 114.241, 59.418 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.456 - 2.037 |
R-factor | 0.1778 |
Rwork | 0.173 |
R-free | 0.22820 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g4b |
RMSD bond length | 0.013 |
RMSD bond angle | 1.320 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.456 | 32.456 | 2.150 |
High resolution limit [Å] | 2.037 | 6.440 | 2.037 |
Rmerge | 0.039 | 0.022 | 0.321 |
Rmeas | 0.044 | ||
Rpim | 0.019 | 0.011 | 0.171 |
Total number of observations | 61150 | 2093 | 6269 |
Number of reflections | 13202 | ||
<I/σ(I)> | 21.2 | 50.9 | 4.2 |
Completeness [%] | 95.5 | 95.7 | 78.3 |
Redundancy | 4.6 | 4.4 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM phosphate-citrate buffer, 40% Peg400, 10% dioxane |